Measurement of C-terminal telopeptide of type I collagen (CTX) in serum

Serum CTX assays measure a fragment of the C-terminal telopeptide of type 1 collagen released during resorption of mature bone. Assay reagents are available in manual and automated formats and give good analytical performance. However their standardisation is not transparent and significant differences in results between methods have been demonstrated. CTX is most stable in EDTA plasma, although serum samples processed promptly would be satisfactory. sCTX shows a profound circadian rhythm, especially in non-fasting subjects; specimens should be collected from fasting patients at a well-defined time of day to minimise biological variation. Reference intervals in pre-menopausal women have been well studied but in other adult groups there is less information. Healthy children show the expected age-related variation corresponding to growth rate. Serum CTX fulfils or partially fulfils all the criteria of a reference bone turnover marker. Further studies aimed at reducing inter-method differences in results and establishing the relationships of sCTX with fracture risk and with fracture risk improvement with treatment are required.

► Serum CTX (sCTX) is a sensitive and specific marker of bone resorption.
► Manual and automated methods are available and perform well analytically.
► Specimens should be collected from fasting patients at a well-defined time.
► Reference intervals for groups other than pre-menopausal women need development.
► sCTX approaches fulfilment of all requirements of a reference bone turnover marker.