A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents

Objectives:To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.Design and methods:Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.Results:Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively.Conclusions:Norfloxacin method is a simple, inexpensive and automatable kinetic assay.