Construction of an amperometric triglyceride biosensor using PVA membrane bound enzymes

ObjectivesA method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes.Design and methodsA mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The biosensor measures current when polarized at 0.4 V.ResultsThe sensor showed optimum response within 2 s  at pH 7.0 and 25 °C. The current (mA) was in proportion to concentration of TG in the range 0.56–2.25 mM. The minimum detection limit of the method was 0.21 mM. The analytic recovery of added TG was 94.3%. Within batch and between batch coefficients of variations (CV) were < 5.85% and < 4.13%, respectively. A good correlation (r =   0.99) was found. Among various serum substances tested, only cholesterol caused slight stimulation (20%).ConclusionsAn amperometric method was developed for determination of TG employing this enzyme electrode.