Oxidative stress and δ-ALA-D activity in different conditioning regimens in allogeneic bone marrow transplantation patients

ObjectivesTo compare different conditioning regimens (CR), in order to determine whether either of them could be less toxic to allogeneic bone marrow transplantation (BMT) patients in terms of oxidative stress and also analyze δ-ALA-D activity as a possible marker of oxidative stress.Design and methodsLipid peroxidation, vitamin C, thiol groups levels and catalase, superoxide dismutase and δ-ALA-D activity were determined in 21 healthy controls, 5 patients with fludarabine + cyclophosphamide (FluCy) CR, 12 with busulfan + cyclophosphamide (BuCy) and 4 with cyclophosphamide + total body irradiation (CyTBI).ResultsThere were a decrease in enzymatic and non enzymatic antioxidants, in δ-ALA-D activity, and in all CRs and an increase in lipid peroxidation more pronounced in CyTBI CR.ConclusionsAll CRs promoted oxidative stress in allogeneic BMT patients, but this was more pronounced with CyTBI and δ-ALA-D activity seemed to be an additional biomarker of oxidative stress in these patients.