Measurement of lysophospholipase D/autotaxin activity in human serum samples

Objectives:Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.Design and methods:LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.Results and conclusions:It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.