کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1971752 1059890 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
AGG interspersion analysis of the FMR1 CGG repeats in mental retardation of unspecific cause
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
AGG interspersion analysis of the FMR1 CGG repeats in mental retardation of unspecific cause
چکیده انگلیسی

ObjectivesTo study the AGG interspersion pattern in mentally retarded patients of unspecified cause.MethodsFMR1 CGG substructure in 104 normal and 232 mentally retarded (MR) males was determined by CGG repeat and AGG interspersion analyses. Genomic DNA of the study subjects was obtained for PCR and Southern hybridization analyses.ResultsAll study subjects had less than 53 CGG repeats and none had fragile X syndrome of mental retardation. There was a significant difference (P < 0.006) in the AGG interspersion pattern. MR males had (1) more variable internal substructures, (2) proportionally less 2 and 3 AGG but more 0 and 1 AGG, less (CGG)9AGG(CGG)9AGG(CGG)9 but more (CGG)9AGG(CGG)19 alleles and (3) a longer pure 3′ CGG repeat.ConclusionsOur results suggest that the MR alleles have a lesser number of interspersed AGG and a longer pure 3′ CGG repeat than the normal population. They are thus more prone to instability and expansion to long repeat lengths as in the fragile X syndrome of mental retardation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Biochemistry - Volume 39, Issue 3, March 2006, Pages 244–248
نویسندگان
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