کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1971900 | 1538992 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Low-intensity pulsed ultrasound induces apoptosis in osteoclasts: Fish scales are a suitable model for the analysis of bone metabolism by ultrasound
ترجمه فارسی عنوان
سونوگرافی پالس با شدت پایین باعث آپوپتوز در استئوکلاست ها می شود: مقیاس های ماهی یک مدل مناسب برای تجزیه و تحلیل متابولیسم استخوان با استفاده از سونوگرافی
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کلمات کلیدی
phorbol-12-myristate-13-acetate-induced protein 1GAPDHCTSKBcl-2TRAPPNPDlx5OCNpara-nitrophenol - para-nitrofenolALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییOsteoblasts - استئوبلاست هاOsteocalcin - استئوکلسین Osteoclasts - استخوانکاه tartrate-resistant acid phosphatase - اسید فسفاتاز مقاوم در برابر تارتاتTdT-mediated dUTP nick end labeling - تلگراف پایان نام نهایی DUTP TdTTUNEL - تونلApoptosis - خزان یاختهایlow-intensity pulsed ultrasound - سونوگرافی پالس با شدت کمB-cell lymphoma 2 - لنفوم سلول B 2LIPUS - لیپوسFish scales - مقیاس های ماهیCathepsin K - کتهفسین کglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Using fish scales in which osteoclasts and osteoblasts coexist on the calcified bone matrix, we examined the effects of low-intensity pulsed ultrasound (LIPUS) on both osteoclasts and osteoblasts. At 3Â h of incubation after LIPUS treatment, osteoclastic markers such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K mRNA expressions decreased significantly while mRNA expressions of osteoblastic markers, osteocalcin, distal-less homeobox 5, runt-related transcription factor 2a, and runt-related transcription factor 2b, increased significantly. At 6 and 18Â h of incubation, however, both osteoclastic and osteoblastic marker mRNA expression did not change at least present conditions. Using GeneChip analysis of zebrafish scales treated with LIPUS, we found that cell death-related genes were upregulated with LIPUS treatment. Real-time PCR analysis indicated that the expression of apoptosis-related genes also increased significantly. To confirm the involvement of apoptosis in osteoclasts with LIPUS, osteoclasts were induced by autotransplanting scales in goldfish. Thereafter, the DNA fragmentation associated with apoptosis was detected in osteoclasts using the TUNEL (TdT-mediated dUTP nick end labeling) method. The multi-nuclei of TRAP-stained osteoclasts in the scales were labeled with TUNEL. TUNEL staining showed that the number of apoptotic osteoclasts in goldfish scales was significantly elevated by treatment with LIPUS at 3Â h of incubation. Thus, we are the first to demonstrate that LIPUS directly functions to osteoclasts and to conclude that LIPUS directly causes apoptosis in osteoclasts shortly after exposure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 195, May 2016, Pages 26-31
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 195, May 2016, Pages 26-31
نویسندگان
Nobuo Suzuki, Taizo Hanmoto, Sachiko Yano, Yukihiro Furusawa, Mika Ikegame, Yoshiaki Tabuchi, Takashi Kondo, Kei-ichiro Kitamura, Masato Endo, Toshio Yamamoto, Toshio Sekiguchi, Makoto Urata, Yuko Mikuni-Takagaki, Atsuhiko Hattori,