کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1972349 | 1060273 | 2012 | 8 صفحه PDF | دانلود رایگان |

Hepatic glycogen synthesis fluxes from direct and indirect pathways were quantified in seabass by postmortem 2H NMR analysis of plasma water (PW) and glycogen glucosyl 2H enrichments from 2H-enriched seawater. Eighteen fish (28.0 ± 1.7 cm and 218.0 ± 43.0 g) were divided into three groups of 6 and studied over 24 days with transfer to 5% 2H-seawater after day 21. Over this period, one group was fed daily with fishmeal, a second group was fasted, and a third group was fasted for 21 days followed by 3 days refeeding. Glycogen turnover and sources were determined from the ratio of glucosyl position 5 enrichment to that of plasma water (H5/PW). Glycogen levels of fed fish were significantly higher than fasted (665.4 ± 345.2 μmol.g− 1 liver versus 77.2 ± 59.5 μmol.g− 1 liver, P < 0.05) while refed fish had comparable levels to fed (584.6 ± 140.4 μmol.g− 1 liver). Glycogen enrichment of fed fish was undetectable indicating negligible turnover over 3 days. For fasted fish, H5/PW was ~ 50% indicating that half of the glycogen had turned over via indirect pathway flux. For refed fish, H5/PW was ~ 100% indicating that the indirect pathway accounted for all net glycogen synthesis. Direct pathway conversion of dietary carbohydrate to glycogen was not detected in any of the groups.
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 163, Issue 1, September 2012, Pages 22–29