کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1977302 | 1539294 | 2014 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Physiological hepatic response to zinc oxide nanoparticle exposure in the white sucker, Catostomus commersonii Physiological hepatic response to zinc oxide nanoparticle exposure in the white sucker, Catostomus commersonii](/preview/png/1977302.png)
Liver toxicity of commercially relevant zinc oxide nanoparticles (nZnO) was assessed in a benthic freshwater cypriniform, the white sucker (Catostomus commersonii). Exposure to nZnO caused several changes in levels of liver enzyme activity, antioxidants, and lipid peroxidation end products consistent with an oxidative stress response. Aconitase activity decreased by ~ 65% but tended to be restored to original levels upon supplementation with Fe2 +, indicating oxidative inactivation of the 4Fe–4S cluster. Furthermore, glucose-6-phosphate dehydrogenase activity decreased by ~ 29%, and glutathione levels increased by ~ 56%. Taken together, these suggest that nZnO induces hepatic physiological stress. Each assay was then validated by using a single liver homogenate or plasma sample that was partitioned and treated with nZnO or Zn2 +, the breakdown product of nZnO. It was found that Zn2 +, but not nZnO, increased detected glutathione reductase activity by ~ 14% and decreased detected malondialdehyde by ~ 39%. This indicates that if appreciable nZnO dissolution occurs in liver samples during processing and assay, it may skew results, with implications not only for this study, but also for a wide range of nanotoxicology studies focusing on nZnO. Finally, in vitro incubations of cell-free rat blood plasma with nZnO failed to generate any significant increase in malondialdehyde or protein carbonyl levels, or any significant decrease in ferric reducing ability of plasma. This suggests that at the level tested, any oxidative stress caused by nZnO is the result of a coordinated physiological response by the liver.
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 162, May 2014, Pages 51–61