کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1977408 1061487 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reciprocal inhibiting interactive mechanism between the estrogen receptor and aryl hydrocarbon receptor signaling pathways in goldfish (Carassius auratus) exposed to 17β-estradiol and benzo[a]pyrene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Reciprocal inhibiting interactive mechanism between the estrogen receptor and aryl hydrocarbon receptor signaling pathways in goldfish (Carassius auratus) exposed to 17β-estradiol and benzo[a]pyrene
چکیده انگلیسی

In the aquatic environment, both the estrogen receptor (ER) and aryl hydrocarbon receptor (AhR) responses are established biomarkers for assessing exposure to pollutants. These receptor responses can also be affected by the presence of other classes of pollutants and may result in misinterpretation of existing pollution. In this study, we investigated the interaction between ER–vitellogenin (VTG) and AhR–cytochrome P450 1A (CYP1A) signaling pathways in goldfish (Carassius auratus) after 10 days exposure to pollutants. 17β-Estradiol (E2) and benzo[a]pyrene (BaP) were selected as the ER and AhR agonists, respectively. The messenger RNA (mRNA) expression of ER–VTG and AhR–CYP1A in liver was determined using quantitative real-time polymerase chain reaction (QRT–PCR). VTG, endogenous E2 and 7-ethoxyresorufin-O-deethylase (EROD) were also studied. Exposure to E2 and BaP alone significantly induced the gene expression of ERα–VTG and AhR2–CYP1A, respectively. Moreover, the obvious expression of related proteins was also observed. However, these inductions were significantly reduced after combined exposure to E2 and lower concentrations of BaP (20 and 50 μg/L), indicative of a reciprocal inhibiting ER–AhR interaction. However, high concentrations (100 μg/L) of BaP did not affect the E2-induced gene expression. Changes in VTG protein were in accordance with the expression of VTG mRNA, and more VTG protein was observed in liver than in serum. The induced endogenous E2 levels were suppressed by the presence of BaP. While the gene expression of CYP1A showed a concentration-dependent increase, EROD induction exhibited a bell-shaped concentration-response curve. Taken together, these results demonstrate a reciprocal inhibiting mode of ER–AhR interactions and may lead to a possible underestimation of actual exposure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 156, Issue 1, June 2012, Pages 17–23
نویسندگان
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