کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1977701 | 1061510 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential transcription of genes involved in peroxisome proliferation in thicklip grey mullets Chelon labrosus injected with benzo(a)pyrene
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Retinoid X receptorDECRPalmitoyl-CoA oxidasePMP70AOX1peroxisome proliferator response elementsMFPRXRCYP1APPARPAHCATAHR3-ketoacyl-CoA thiolase - 3-ketoacyl-CoA تیولازB(a)P - B (a) PROS - ROSPPRE - ارسالBenzo(a)pyrene - بنزو (a) پیرنهPeroxisome proliferation - ترویج پراکسیومThio - تیوcytochrome P450 1a - سیتوکروم P450 1aPolycyclic aromatic hydrocarbon - هیدروکربن آروماتیک چند حلقه ایMultifunctional protein - پروتئین چند منظورهChelon labrosus - چلون لابروزCatalase - کاتالازReactive oxygen species - گونههای فعال اکسیژنaryl hydrocarbon receptor - گیرنده آرویل هیدروکربنPeroxisome proliferator activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Differential transcription of genes involved in peroxisome proliferation in thicklip grey mullets Chelon labrosus injected with benzo(a)pyrene Differential transcription of genes involved in peroxisome proliferation in thicklip grey mullets Chelon labrosus injected with benzo(a)pyrene](/preview/png/1977701.png)
چکیده انگلیسی
Benzo(a)pyrene (B(a)P) is a mutagenic polycyclic aromatic hydrocarbon (PAH) commonly released into the environment. B(a)P induces phase I biotransformation metabolism and peroxisome proliferation which is characterised in rodents by increased peroxisomal volume density, accompanied by the transcriptional induction of peroxisomal proteins. The aim of the present work was to study peroxisome proliferation at the transcriptional level, in comparison to the transcription of the well-characterised cytochrome P450 1A gene (cyp1a) in the thicklip grey mullet Chelon labrosus. For this purpose, genes coding for the major peroxisomal membrane protein PMP70 and CYP1A were cloned using degenerate primers. Then juvenile mullets were single injected with B(a)P (5 mg/kg) and transcription of palmitoyl-CoA oxidase (aox1), multifunctional protein (mfp1), 3-ketoacyl-CoA thiolase (thio), Î2,Î4dienoyl-CoA reductase 2, pmp70, catalase and cyp1a was semi-quantified in liver and gills 1 and 7 days after the injection. Transcription of aox1 and cyp1a was induced in gills 1 day after B(a)P injection. Cyp1a transcription was also induced in mullet liver one day after injection, indicating that B(a)P was available for the liver. This was further proved by the significant accumulation of B(a)P-like metabolites in bile 7 days after the injection. In liver, aox1, mfp1 and thio transcription was induced at day 1 followed by the induction of catalase transcription at day 7 that may reflect a response to an oxidative stress caused by B(a)P itself or by oxyradicals produced through the biotransformation metabolism and the peroxisomal β-oxidation. These hepatic responses were not reflected at AOX1 activity level. In conclusion, it has been shown for the first time that the three enzymes in the fish peroxisomal β-oxidation pathway are transcriptionally induced by B(a)P. It remains to be tested whether this enhanced transcription is reflected in an increase in the volume of peroxisomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 151, Issue 3, April 2010, Pages 334-342
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 151, Issue 3, April 2010, Pages 334-342
نویسندگان
Eider Bilbao, Miren P. Cajaraville, Ibon Cancio,