کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1978241 1061534 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Endocrine modulating actions of a phytosterol mixture and its oxidation products in zebrafish (Danio rerio)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Endocrine modulating actions of a phytosterol mixture and its oxidation products in zebrafish (Danio rerio)
چکیده انگلیسی

In this study, a phytosterol preparation (“ultrasitosterol”; 80% β-sitosterol) and an oxidized ultrasitosterol preparation were evaluated for reproductive effects in zebrafish. Adults were exposed in a continuous flow to 10 μg/L and 100 μg/L ultrasitosterol and oxidized ultrasitosterol, and to 0.27 μg/L 17β-estradiol and 0.28 μg/L testosterone for 3 weeks. Biomarkers analysed included plasma vitellogenin, testosterone, 11-ketotestosterone, 17β-estradiol, and gonadal histopathology. Ovarian steroid production of testosterone and 17β-estradiol was examined in isolated zebrafish follicles exposed in vitro to the compounds at the same concentrations as in vivo. Vtg was induced in males exposed to ultrasitosterol, and in males and females exposed to 17β-estradiol. Males exposed to oxidized phytosterols showed increased levels of testosterone and 11-ketotestosterone, and accelerated spermatogenesis. Increased follicular atresia was observed in females exposed to oxidized phytosterols and 17β-estradiol. Correlation analyses between biomarkers revealed more intercorrelated values for females than for males, and the strongest associations were found in females exposed to oxidized phytosterols. Testosterone production was significantly increased in follicles exposed to the oxidized phytosterol preparations. These findings indicate that the phytosterol mixture is weakly estrogenic in male fish, and that the oxidized phytosterol mixture contains substances that may interfere with spermatogenesis, oogenesis and gonadal steroidogenesis in zebrafish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 145, Issue 4, May 2007, Pages 518–527
نویسندگان
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