کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985602 1540229 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rheological, biocompatibility and osteogenesis assessment of fish collagen scaffold for bone tissue engineering
ترجمه فارسی عنوان
ارزیابی زیستشناسی، بیولوژیک و پوکی استخوان از داربست کلاژن ماهی برای مهندسی بافت استخوان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Addition of chitosan had improved the mechanical properties of scaffold.
• Higher level of ALP induced by collagen-hydroxyapatite scaffold.
• Biodegradation rate of scaffold was increased by addition of chitosan.

In the present investigation, an attempt was made to find an alternative to mammalian collagen with better osteogenesis ability. Three types of collagen scaffolds – collagen, collagen-chitosan (CCH), and collagen-hydroxyapatite (CHA) – were prepared from the cartilage of Blue shark and investigated for their physico-functional and mechanical properties in relation to biocompatibility and osteogenesis. CCH scaffold was superior with pH 4.5–4.9 and viscosity 9.7–10.9 cP. Notably, addition of chitosan and HA (hydroxyapatite) improved the stiffness (11–23 MPa) and degradation rate but lowered the water binding capacity and porosity of the scaffold. Interestingly, CCH scaffolds remained for 3 days before complete in-vitro biodegradation. The decreased amount of viable T-cells and higher level of FAS/APO-1 were substantiated the biocompatibility properties of prepared collagen scaffolds. Osteogenesis study revealed that the addition of CH and HA in both fish and mammalian collagen scaffolds could efficiently promote osteoblast cell formation. The ALP activity was significantly high in CHA scaffold–treated osteoblast cells, which suggests an enhanced bone-healing process. Therefore, the present study concludes that the composite scaffolds prepared from fish collagen with higher stiffness, lower biodegradation rate, better biocompatible, and osteogenesis properties were suitable biomaterial for a bone tissue engineering application as an alternative to mammalian collagen scaffolds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 91, October 2016, Pages 51–59
نویسندگان
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