Chemical characterization of heteropolysaccharides from green and black teas (Camellia sinensis) and their anti-ulcer effect

• New heterpolysaccharides from green and black teas from Camellia sinensis.
• An arabinogalactan-protein from green and black teas alkaline extract had its fine chemical structure first described.
• An heteroxylan from green and black teas alkaline extract were for first time identified from C. sinensis.
• The heteropolysaccharides presented a significant gastroprotective action.
• The mechanism of gastroprotection was maintenance of gastric mucus and GSH.

In order to obtain polysaccharides from green and black teas (Camellia sinensis), commercial leaves were submitted to infusion and then to alkaline extraction. The extracts were fractionated by freeze-thawing process, giving insoluble and soluble fractions. Complex arabinogalactan protein from the soluble fractions of both teas (GTPS and BTPS) were determined by methylation analysis and 1H/13C-HSQC spectroscopy, showing a main chain of (1 → 3)-β-Galp, substituted at O-6 by (1 → 6)-linked β-Galp with side chains of α-Araf and terminal units of α-Araf, α-Fucp and α-Rhap. A highly branched heteroxylan from the insoluble fractions (GTPI and BTPI) showed in methylation analysis and 1H/13C-HSQC spectroscopy the main chain of (1 → 4)-β-Xylp, substituted in O-3 by α-Araf, β-Galp and α-Glcp units. Evaluating their gastroprotective activity, the fractions containing the soluble heteropolysaccharides from green (GTPS) and black teas (BTPS) reduced the gastric lesions induced by ethanol. Furthermore, the fraction of insoluble heteropolysaccharides of green (GTPI) and black (BTPI) teas also protected the gastric mucosa. In addition, the maintenance of gastric mucus and reduced glutathione (GSH) levels was involved in the polysaccharides gastroprotection.