کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1986310 1540250 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Covalent attachment of lipases on glyoxyl-agarose beads: Application in fruit flavor and biodiesel synthesis
ترجمه فارسی عنوان
پیوستگی کووالانتی لیپازها در دانههای گلیوکسیل آگاروز: کاربرد در طعم میوه و سنتز بیودیزل
کلمات کلیدی
بی حرکت سازی لیپاز، دانه های گلایکسی آگارز، سنتز استر
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

The aim of this work was to prepare biocatalysts to catalyze the synthesis of butyl butyrate by esterification reaction, and the synthesis of biodiesel by transesterification of palm and babassu oils with ethanol. Lipase preparations Lipolase® (TLL1) and Lipex® 100L (TLL2) from Thermomyces lanuginosus and Lipase AK from Pseudomonas fluorescens (PFL) were immobilized on glyoxyl-agarose beads prepared by activation with glycidol (Gly) and epichlorohydrin (Epi). The influence of immobilization time, lipase source and activating agents on the catalytic activity of the biocatalysts were evaluated in both aqueous and organic media. TLL1 immobilized on glyoxyl-agarose by 24 h of incubation resulted biocatalysts with high hydrolytic activity (varying from 1347.3 to 1470.0 IU/g of support) and thermal-stability, around 300-fold more stable than crude TLL1 extract. The maximum load of immobilized TLL1 was around 20 mg of protein/g of support. The biocatalyst prepared exhibited high activity and operational stability on the butyl butyrate synthesis by esterification after five successive cycles of 24 h each (conversion around 85–90%). Immobilized TLL1 and PFL were active in the synthesis of biodiesel by transesterification reaction. Maximum transesterification yield (≥98.5% after 48 h of reaction at 45 °C) was provided by using palm oil as feedstock.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 70, September 2014, Pages 78–85
نویسندگان
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