کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1986349 | 1540250 | 2014 | 6 صفحه PDF | دانلود رایگان |
• Structural characterization of water-soluble polysaccharides produced by P. florida mycelia.
• MG-PfM – α-d-Galp and 3-O-Me-d-Galp units (1 → 6)-linked, highly substituted at O-2 by D-Manp.
• GLY-PfM – α-d-Glp (1 → 4)-linked, partially substituted at O-6 by α-d-Glcp side chains.
• βGLC-PfM – β-d-Glcp (1 → 3), (1 → 6)-linked, with β-d-Glcp (1 → 6)-linked side chains.
• It is possible to obtain bioactive molecules from submerged culture.
Pleurotus ostreatus var. florida known as Hiratake has a high nutritional value, presents medicinal and nutraceutical properties and it is one of the consumed mushrooms in Brazil. Thus, the aim of this study was to characterize the chemical structure of polysaccharides found in mycelial biomass produced by submerged culture of P. ostreatus var. florida in order to compare with those found in P. ostreatus var. florida fruit bodies. Aqueous and alkali extracts obtained from mycelial biomass were purified, 13C NMR, GC–MS and chemical techniques were used to characterize three polysaccharide structures: a mannogalactan (MG-PfM) with α-d-Galp and 3-O-Me-α-d-Galp units, both (1 → 6)-linked, highly substituted at O-2 by D-Manp, a glycogen-like polymer (GLY-PfM) with α-d-Glp (1 → 4)-linked main chain, partially substituted at O-6 by α-d-Glcp side chains and a (1 → 3), (1 → 6) β-d-glucan (βGLC-PfM) with a main chain of β-d-Glcp (1 → 3)-linked units, partially substituted at O-6 by side chains of 6-O-substituted β-d-glucopyranosyl units, on an average of one to every two residues of the backbone. These results show the possibility to obtain similar and also different molecules from those found in the fruiting body of the same mushroom species, therefore the submerged culture of mushroom is a promising way to give raise molecules of interest.
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Journal: International Journal of Biological Macromolecules - Volume 70, September 2014, Pages 354–359