کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1986607 1540256 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biophysical characterization of in vitro bound Streptomyces peucetius daunorubicin–serine protease complex
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Biophysical characterization of in vitro bound Streptomyces peucetius daunorubicin–serine protease complex
چکیده انگلیسی

A serine protease of Streptomyces peucetius is found in association with daunorubicin in the culture filtrate and co-purifies as a complex as reported earlier by us (Dubey et al., 2013). The same protease was purified without drug attachment from dpsA− mutant of S. peucetius, which does not produce daunorubicin. Drug–protein complex was made in vitro by mixing daunorubicin and the protease. Spectral analysis and circular dichroism (CD) analysis were employed to determine the interaction between daunorubicin and the protease. Our study showed that interaction of daunorubicin with the protease affects the spectral characteristics of the drug and changes the secondary structure of the protein. Thin layer chromatography (TLC) analysis showed that the drug–protein interaction results in partial conversion of the drug to aglyconic form. The complex formation implies sequestration of the drug when it attains potentially lethal level in the extracellular milieu of S. peucetius culture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 64, March 2014, Pages 111–114
نویسندگان
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