کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1986770 1540259 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of neuropeptide loaded trimethyl chitosan nanoparticles for nose to brain delivery
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Evaluation of neuropeptide loaded trimethyl chitosan nanoparticles for nose to brain delivery
چکیده انگلیسی
Leucine-enkephalin (Leu-Enk) is a neurotransmitter or neuromodulator in pain transmission. Due to non-addictive opioid analgesic activity of this peptide, it might have great potential in pain management. Leu-Enk loaded N-trimethyl chitosan (TMC) nanoparticles were prepared and evaluated as a brain delivery vehicle via nasal route. TMC biopolymer was synthesized and analyzed by 1H NMR spectroscopy. TMC nanoparticles were prepared by ionic gelation method. Mean peptide encapsulation efficiency and loading capacity were 78.28 ± 3.8% and 14 ± 1.3%, respectively. Mean particle size, polydispersity index and zeta potential were found to be 443 ± 23 nm, 0.317 ± 0.17 and +15 ± 2 mV respectively for optimized formulations. Apparent permeability coefficient (Papp) of Leu-Enk released from nanoparticles across the porcine nasal mucosa was determined to be 7.45 ± 0.30 × 10−6 cm s−1. Permeability of Leu-Enk released from nanoparticles was 35 fold improved from the nasal mucosa as compared to Leu-Enk solution. Fluorescent microscopy of brain sections of mice showed higher accumulation of fluorescent marker NBD-F labelled Leu-Enk, when administered nasally by TMC nanoparticles, while low brain uptake of marker solution was observed. Furthermore, enhancement in brain uptake resulted into significant improvement in the observed antinociceptive effect of Leu-Enk as evidenced by hot plate and acetic acid induced writhing assay.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 61, October 2013, Pages 189-195
نویسندگان
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