کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987128 1540293 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Dietary bioflavonoids inhibit Escherichia coli ATP synthase in a differential manner
چکیده انگلیسی

The aim of this study was to determine if the dietary benefits of bioflavonoids are linked to the inhibition of ATP synthase. We studied the inhibitory effect of 17 bioflavonoid compounds on purified F1 or membrane bound F1FoE. coli ATP synthase. We found that the extent of inhibition by bioflavonoid compounds was variable. Morin, silymarin, baicalein, silibinin, rimantadin, amantidin, or, epicatechin resulted in complete inhibition. The most potent inhibitors on molar scale were morin (IC50 ∼ 0.07 mM) > silymarin (IC50 ∼ 0.11 mM) > baicalein (IC50 ∼ 0.29 mM) > silibinin (IC50 ∼ 0.34 mM) > rimantadin (IC50 ∼ 2.0 mM) > amantidin (IC50 ∼ 2.5 mM) > epicatechin (IC50 ∼ 4.0 mM). Inhibition by hesperidin, chrysin, kaempferol, diosmin, apigenin, genistein, or rutin was partial in the range of 40–60% and inhibition by galangin, daidzein, or luteolin was insignificant. The main skeleton, size, shape, geometry, and position of functional groups on inhibitors played important role in the effective inhibition of ATP synthase. In all cases inhibition was found fully reversible and identical in both F1Fo membrane preparations and isolated purified F1. ATPase and growth assays suggested that the bioflavonoid compounds used in this study inhibited F1-ATPase as well as ATP synthesis nearly equally, which signifies a link between the beneficial effects of dietary bioflavonoids and their inhibitory action on ATP synthase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 46, Issue 5, 1 June 2010, Pages 478–486
نویسندگان
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