کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987369 1540286 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characteristics of sulfated polysaccharides from Monostroma nitidum and their in vitro anticancer and immunomodulatory activities
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Molecular characteristics of sulfated polysaccharides from Monostroma nitidum and their in vitro anticancer and immunomodulatory activities
چکیده انگلیسی

We investigated water-soluble sulfated polysaccharides isolated from Monostroma nitidum using ion-exchange chromatography to determine their molecular characteristics and biological activities. The crude and fractionated polysaccharides (F1, F2, and F3) consisted mostly of carbohydrates (58.3–91.9%), uronic acids (0–21.8%) and sulfates (1.8–17.7%) as well as varying amounts of proteins (1.6–9.4%). Their monosaccharide levels were significantly different including rhamnose (0–95.7%) and glucose (0–98.6%) content with small amounts of xylose (0.8–4.3%). These polysaccharides contained one or two subfractions with average molecular weights (Mw) ranging from 94.4 to 1387 × 103 g/mol. The in vitro inhibitory activity (≤75%) of the polysaccharides on a human cancer cell line (AGS) suggested that the polysaccharides had direct cytotoxic effects on the cancer cells. In addition, these hetero-polysaccharides (from the crude and F1 and F2 fractions) stimulated a macrophage cell line, Raw 264.7 cells, inducing considerable NO and PGE2, production, which suggested that they could be strong immunomodulators.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 48, Issue 2, 1 March 2011, Pages 311–318
نویسندگان
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