کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1987456 1540288 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Liposomes incorporating hydrophobically modified silk fibroin: pH-dependent release
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Liposomes incorporating hydrophobically modified silk fibroin: pH-dependent release
چکیده انگلیسی

pH-sensitive liposomes were prepared by modifying the surface of egg phosphatidylcholine (EPC) liposomes with hydrophobically modified silk fibroin (HmSF). For the hydrophobic modification of SF, palmitic acid residues were covalently attached to SF through amide bond. According to the result of TNBS assay, about 90% of amino groups of SF participated in the conjugation reaction with palmitic acid N-hydroxysuccinimide ester, and the number of palmitic acid residue per SF molecule was calculated to be 15.3. The fluorescence quenching of calcein in liposomal suspension decreased from about 80–50%, when the ratio of HmSF to phospholipid increased from 1:20 to 1:5. The maximum degree of release from liposomes incorporating HmSF (of which HmSF to phospholipid ratio was 1:20) for 5 min was about 95% at pH 4.5, 25% at pH 5.0, and 5% at pH 5.5, and no appreciable amount of release was observed in the range of pH 6.5 to pH 8.0. Under acidic conditions, HmSF is likely to perturb the packing of phospholipid in liposomal membrane, possibly due to the unionization of the carboxylic groups of the silk protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 47, Issue 5, 1 December 2010, Pages 635–639
نویسندگان
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