کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988251 1540318 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding
چکیده انگلیسی

The embryo of oviparous species is confronted by a highly oxidative stress generating as it grows and must rely on effective antioxidant system for protection. Proteins of avian egg albumin have been suggested to play the major redox-modulatory role during embryo development. Recently, we found that ovotransferrin (OTf) undergoes distinct thiol-linked self-cleavage in a redox-dependent process. In this study, we explore that OTf is SOD mimic protein with a potent superoxide anion (O2−) scavenging activity. The O2− scavenging activity was investigated using the natural xanthine/xanthine oxidase (X/XOD) coupling system. OTf exhibited O2− scavenging activity in a dose-dependent manner and showed remarkably higher scavenging activity than the known antioxidants, ascorbate or serum albumin. The isolated half-molecules of OTf exhibited higher scavenging activity than the intact molecule, whereas the N-lobe showed much greater activity. OTf dramatically quenched the O2− flux but had no effect on the urate production in the X/XOD system, indicating its unique specificity to scavenge O2− but not oxidase inhibition. Strikingly, metal-bound OTf exhibited greater O2− dismutation capacity than the apo-protein, ranging from moderate (Zn2+-OTf and Fe2+-OTf) to high (Mn2+-OTf and Cu2+-OTf) activity with the Cu2+-OTf being the most potent scavenger. In a highly sensitive fluorogenic assay, the metal-bound OTf exhibited significant increase in the rate of H2O2 production in the X/XOD reaction than the apo-OTf, providing evidence that Zn2+-, Mn2+- and Cu2+-OTf possess SOD mimic activity. This finding is the first to describe that OTf is an O2− scavenging molecule, providing insight into its novel SOD-like biological function, and heralding a fascinating opportunity for its potential candidacy as antioxidant drug.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 41, Issue 5, 1 December 2007, Pages 631–640
نویسندگان
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