کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988711 1540445 2016 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells
ترجمه فارسی عنوان
القایی از سلول های بنیادی موش بالغ استخوان مزانشیمی مغز به سلولهای عصبی حرکتی عملکردی
کلمات کلیدی
سلول های بنیادی مزانشیمی؛ نورون حرکتی؛ استیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol.
• Differentiation confirmed using light, electron microscope and Immunocytochemistry.
• Cells were function as motor neuron as assessed by measuring acetylcholine by HPLC.
• Motor neuron axon elongated by nerve growth factor.
• Using a 3D collagen matrix showed that cells were induced into motor neuron.

The differentiation of mesenchymal stem cells (MSC) into acetylcholine secreted motor neuron-like cells, followed by elongation of the cell axon, is a promising treatment for spinal cord injury and motor neuron cell dysfunction in mammals. Differentiation is induced through a pre-induction step using Beta- mercaptoethanol (BME) followed by four days of induction with retinoic acid and sonic hedgehog. This process results in a very efficient differentiation of BM-MSCs into motor neuron-like cells. Immunocytochemistry showed that these treated cells had specific motor neural markers: microtubule associated protein-2 and acetylcholine transferase. The ability of these cells to function as motor neuron cells was assessed by measuring acetylcholine levels in a culture media during differentiation. High-performance liquid chromatography (HPLC) showed that the differentiated cells were functional. Motor neuron axon elongation was then induced by adding different concentrations of a nerve growth factor (NGF) to the differentiation media. Using a collagen matrix to mimic the natural condition of neural cells in a three-dimensional model showed that the MSCs were successfully differentiated into motor neuron-like cells. This process can efficiently differentiate MSCs into functional motor neurons that can be used for autologous nervous system therapy and especially for treating spinal cord injuries.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 77, November 2016, Pages 129–142
نویسندگان
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