کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1988711 | 1540445 | 2016 | 14 صفحه PDF | دانلود رایگان |
• Efficient differentiation of BM-MSCs into motor neuron-like cells revised protocol.
• Differentiation confirmed using light, electron microscope and Immunocytochemistry.
• Cells were function as motor neuron as assessed by measuring acetylcholine by HPLC.
• Motor neuron axon elongated by nerve growth factor.
• Using a 3D collagen matrix showed that cells were induced into motor neuron.
The differentiation of mesenchymal stem cells (MSC) into acetylcholine secreted motor neuron-like cells, followed by elongation of the cell axon, is a promising treatment for spinal cord injury and motor neuron cell dysfunction in mammals. Differentiation is induced through a pre-induction step using Beta- mercaptoethanol (BME) followed by four days of induction with retinoic acid and sonic hedgehog. This process results in a very efficient differentiation of BM-MSCs into motor neuron-like cells. Immunocytochemistry showed that these treated cells had specific motor neural markers: microtubule associated protein-2 and acetylcholine transferase. The ability of these cells to function as motor neuron cells was assessed by measuring acetylcholine levels in a culture media during differentiation. High-performance liquid chromatography (HPLC) showed that the differentiated cells were functional. Motor neuron axon elongation was then induced by adding different concentrations of a nerve growth factor (NGF) to the differentiation media. Using a collagen matrix to mimic the natural condition of neural cells in a three-dimensional model showed that the MSCs were successfully differentiated into motor neuron-like cells. This process can efficiently differentiate MSCs into functional motor neurons that can be used for autologous nervous system therapy and especially for treating spinal cord injuries.
Journal: Journal of Chemical Neuroanatomy - Volume 77, November 2016, Pages 129–142