کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2006922 | 1066358 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation, identification and biological activity of gastrin-releasing peptide 1-46 (oGRP1-46), the primary GRP gene-derived peptide product of the pregnant ovine endometrium
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کلمات کلیدی
small cell lung cancer cellsbombesin receptor subtype 3MALDI-TOFBRS-3neuromedin B receptorHRPNMBRNaN3TFARP-HPLCGRPRRIABSA - BSAC-terminus - C- ترمینالbovine serum albumin - آلبومین سرم گاوEndometrium - آندومترTrifluoroacetic acid - اسید TrifluoroaceticPregnancy - بارداریSCLC - بگذارندOvine - تخم مرغcarboxyl terminus - ترمینال کربوکسیلCNS - دستگاه عصبی مرکزیradioimmunoassay - رادیوایمونواسیsodium azide - سدیم آیزیدcentral nervous system - سیستم عصبی مرکزیMatrix assisted laser desorption ionization time-of-flight - ماتریکس از زمان پرواز یونیزاسیون لیزر استفاده کردHorseradish peroxidase - پراکسیداز هوررادیشPeptide - پپتید gastrin-releasing peptide - پپتید آزاد کننده گاسترینreverse-phase high pressure liquid chromatography - کروماتوگرافی مایع با فشار بالا فاز معکوسgastrin-releasing peptide receptor - گیرنده پپتید آزاد کننده گاسترین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have previously demonstrated that pregnant ovine endometrium expresses the gastrin-releasing peptide (GRP) gene at a high level following conceptus implantation. Here we report the isolation, characterization and biological activity of ovine GRP1-46, the primary product of this gene in the pregnant endometrium. Full thickness 125-140-day pregnant sheep uterus (term is 145 day) was homogenized in 80% acetonitrile/2% trifluoroacetic acid (1:7 ACN/TFA), concentrated on reverse-phase C18 cartridges and chromatographed successively on gel filtration (Sephadex G-50) and reverse-phase HPLC (C18 μBondapak). Purification was monitored by RIA. Purified GRP peptide was analysed by mass spectrometry giving a major mass ion at 4963 which corresponds exactly to GRP1-46. Other mass ions from pro-GRP did not contain a biologically active N-terminus or antigenic determinant. Proteolytic cleavage of pro-GRP to give rise to GRP1-46 would require preferential cleavage at the Glu-Glu bond by a Glu-C2-like enzyme, rather than the trypsin-like and C-terminal amidation enzymes (PAM) that produce GRP18-27 and GRP1-27 in other tissues. GRP1-46 was synthesized and receptor binding and biological activity tested on a range of rodent and human cell lines that express GRP-related receptors GRPR, NMBR and BRS3. GRP1-46 bound GRPR and NMBR with low affinity, and mobilized inositol phosphate in cell lines expressing the GRPR and NMBR, but not BRS-3. This study describes a new processed product of the GRP gene, GRP1-46, which is highly expressed in the pregnant sheep endometrium and which acts as a weak agonist at the GRPR and NMBR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 31, Issue 2, February 2010, Pages 284-290
Journal: Peptides - Volume 31, Issue 2, February 2010, Pages 284-290
نویسندگان
A.S. Giraud, C. Dumesny, J.C. Whitley, L.M. Parker, I. Jennings, B. Kemp, T.W. Moody, V. Sancho, R.T. Jensen, A. Shulkes,