کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2007445 1066375 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improvement of lipoxygenase inhibition by octapeptides
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Improvement of lipoxygenase inhibition by octapeptides
چکیده انگلیسی

The β-casein-derived octapeptide RINKKIEK is a noncompetitive inhibitor of soybean lipoxygenase (LOX). To investigate the molecular determinants for the enzyme–peptide interaction, a peptide library containing substitutional analogs of RINKKIEK was prepared by SPOT synthesis and analyzed for interaction with fluorescent-labeled LOX. The positively charged amino acid residues in RINKKIEK appear to be essential for the LOX–peptide interaction. Replacement of the negatively charged glutamic acid by any other amino acid residue improves LOX binding. For both RINKKIPK and RINKKISK this increase in LOX binding is accompanied by a threefold increase in LOX inhibition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 28, Issue 12, December 2007, Pages 2268–2275
نویسندگان
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