کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2008042 1066395 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples: Identification of novel brevinins from three species of Chinese frogs
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples: Identification of novel brevinins from three species of Chinese frogs
چکیده انگلیسی

Brevinins are peptides of 24 amino acid residues, originally isolated from the skin of the Oriental frog, Rana brevipoda porsa, by nature of their microbicidal activity against a wide range of Gram-positive and Gram-negative bacteria and against strains of pathogenic fungi. cDNA libraries were constructed from lyophilized skin secretion of three, unstudied species of Chinese frog, Odorrana schmackeri, Odorrana versabilis and Pelophylax plancyi fukienensis, using our recently developed technique. In this report, we describe the “shotgun” cloning of novel brevinins by means of 3′-RACE, using a “universal” degenerate primer directed towards a highly conserved nucleic acid sequence domain within the 5′-untranslated region of previously characterized frog skin peptide cDNAs. Novel brevinins, deduced from cloned cDNA open-reading frames, were subsequently identified as mature peptides in the same samples of respective species skin secretions. Bioinformatic analysis of both prepro-brevinin nucleic acid sequences and translated open-reading frame amino acid sequences revealed a highly conserved signal peptide domain and a hypervariable anti-microbial peptide-encoding domain. The experimental approach described here can thus rapidly provide robust structural data on skin anti-microbial peptides without harming the donor amphibians.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 27, Issue 1, January 2006, Pages 42–48
نویسندگان
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