کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
200853 | 1424353 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts](/preview/png/200853.png)
BackgroundFollistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN).ResultsThe duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in these cells significantly inhibited the mRNA expression of MSTN (a target gene that is negatively regulated by FST).ConclusionsThe duck pEGFP-FST vector has been constructed successfully and exhibits biological activity by promoting myoblast proliferation and differentiation in vitro.
Journal: Electronic Journal of Biotechnology - Volume 17, Issue 5, September 2014, Pages 224–229