Effect of dimethoate and UV-B irradiation on the response of antioxidant defense systems in cowpea (Vigna unguiculata L.) seedlings

To understand the effect of pesticide and UV-B on plant non-enzymatic antioxidant system, Vigna unguiculata was exposed to different concentration of dimethoate and UV-B (30 min) alone and in combination. Negative impact on V. unguiculata   due to generation of superoxide radical (O2-), hydrogen peroxide (H2O2), electrolyte leakage (EC) and lipid peroxidation (MDA) was observed. Combined exposure to UV-B and dimethoate led to further increase in these parameters. The content of non-enzymatic antioxidants such as proline and flavonoids increased whereas the ascorbic acid content showed a decrease. The results suggested the role of proline and flavonoids in scavenging reactive oxygen species generated due to stress.

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► Pesticide and UV-B effect on plant non-enzymatic antioxidant system of Vigna unguiculata.
► Generation of ROS, electrolyte leakage and lipid peorxidation leads to adverse impacts.
► Proline and flavonoids play a key role in scavenging ROS generated due to stress.