Characterization of a Toxoneuron nigriceps (Viereck) (Hymenoptera: Braconidae) - derived chitinase and its potential for pest control

► A chitinase of Toxoneuron nigriceps (TnChi) was expressed in tobacco plants. ► The binding efficiency of Tnchi to chitin is pH dependent. ► Tnchi lacks chitinolytic activity, but has insecticidal activity. ► Bioassays and bioinformatics indicated Tnchi as an inactive chitotriosidase with chitin binding properties.