کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2025415 1069995 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct detection of soil mRNAs using targeted microarrays for genes associated with lignin degradation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Direct detection of soil mRNAs using targeted microarrays for genes associated with lignin degradation
چکیده انگلیسی

Microarrays have become established tools for describing microbial systems, however the direct assessment of expression profiles for uncharacterized environmental microbial communities still presents unique challenges. Notably, the concentration of particular transcripts are likely very dilute relative to the pool of total RNA, and PCR-based amplification strategies are vulnerable to amplification biases and the appropriate primer selection. Thus we applied a target labeling and amplification approach based on the Klenow fragment and signal amplification approach to detect expression of fungally-derived lignin-degrading enzymes in soil. Known amplicons and cDNA from Phanerochaete chrysosporium were mixed with the soil cDNA both before and after the signal amplification in order to assess the dynamic range of the microarray. The addition of control cDNA with soil cDNA interfered with detection of the low-abundance transcripts. Nevertheless this microarray approach consistently reported the higher-abundance transcripts which presented more robust signals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 42, Issue 10, October 2010, Pages 1793–1799
نویسندگان
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