کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2025763 1070009 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stress response of Burkholderia cepacia WZ1 exposed to quinclorac and the biodegradation of quinclorac
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
پیش نمایش صفحه اول مقاله
Stress response of Burkholderia cepacia WZ1 exposed to quinclorac and the biodegradation of quinclorac
چکیده انگلیسی

Quinclorac is an effective herbicide, but its residue is known to be a major environmental pollutant. Therefore, isolating quinclorac-degrading bacteria, exploring the mechanisms of degradation and analyzing the proteins introduced by the pollutant, would have scientific value and practical importance. We used quinclorac-degrading bacteria WZ1 (an opportunistic pathogen with broad substrate characteristic) as the test strain, and used several techniques such as two-dimensional electrophoresis, gas chromatography–mass spectrometry and Northern Blotting to study the stress response of the strain upon environmental stress and the biodegradation of quinclorac. The results showed that strain WZ1 secreted some protective proteins such as Mn-SOD, Heat-shock protein and Taurine dioxygenase to deal with the stress of quinclorac; while the expression of proteins DsbA and Gmet_1484 related to bacterial pathogenesis decreased significantly when exposed to quinclorac (which could be used to indicate that bacterial pathogenicity was attenuated). At the same time, quinclorac also induced the expression of phthalate dioxygenase reductase and chlorocatechol 1, 2-dioxygenase. They are the key proteins participating in the degradation of quinclorac, which was proved by the Northern Blotting. With the information gathered, we attempted to define the degradation pathway of quinclorac.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 41, Issue 5, May 2009, Pages 984–990
نویسندگان
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