کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2027670 1542695 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors
ترجمه فارسی عنوان
تکامل خاصیت کورتیکواستروئید برای گیرنده های گلوکوکورتیکوئید انسان، مرغ، آلیاژاتور و قورباغه
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Response to corticosteroids evolved in terrestrial vertebrate GRs.
• Allosteric interactions between A/B/C and hinge/LBD domains regulate GR response.
• Different responses to steroids for full length and GAL4-DBD-hinge-LBD GRs.

We investigated the evolution of the response of human, chicken, alligator and frog glucocorticoid receptors (GRs) to dexamethasone, cortisol, cortisone, corticosterone, 11-deoxycorticosterone, 11-deoxycortisol and aldosterone. We find significant differences among these vertebrates in the transcriptional activation of their full length GRs by these steroids, indicating that there were changes in the specificity of the GR for steroids during the evolution of terrestrial vertebrates. To begin to study the role of interactions between different domains on the GR in steroid sensitivity and specificity for terrestrial GRs, we investigated transcriptional activation of truncated GRs containing their hinge domain and ligand binding domain (LBD) fused to a GAL4 DNA binding domain (GAL4-DBD). Compared to corresponding full length GRs, transcriptional activation of GAL4-DBD_GR-hinge/LBD constructs required higher steroid concentrations and displayed altered steroid specificity, indicating that interactions between the hinge/LBD and other domains are important in glucocorticoid activation of these terrestrial GRs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 113, September 2016, Pages 38–45
نویسندگان
, , , ,