Steroidal glycosides from the bulbs of Fritillaria meleagris and their cytotoxic activities

• 10 New steroidal glycosides were isolated from the bulbs of Fritillaria meleagris.
• Apoptosis in HL-60 and A549 were evaluated by caspase-3 and flow cytometry.
• 5β-spirostanol (2) and a cholestane (17a) induced apoptosis through different mechanisms.
• (22R)-spirosolanol (11) selectively induced apoptosis without affecting caspase-3.

Steroidal glycosides (1–18), including 10 new compounds (1–10), were isolated from the bulbs of Fritillaria meleagris (Liliaceae). The structures of the new compounds were determined by two-dimensional (2D) NMR analysis, and by hydrolytic cleavage followed by spectroscopic and chromatographic analysis. The isolated compounds and their aglycones were evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung adenocarcinoma cells. Morphological observation and flow cytometry analysis showed that 5β-spirostanol glycoside (2) and a cholestane derivative (17a) induced apoptotic cell death in HL-60 cells through different mechanisms of action. Furthermore, the (22R)-spirosolanol glycoside (11) selectively induced apoptosis in A549 cells without affecting the caspase-3 activity level.

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