کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2028016 | 1070388 | 2013 | 13 صفحه PDF | دانلود رایگان |
• 10 New steroidal glycosides were isolated from the bulbs of Fritillaria meleagris.
• Apoptosis in HL-60 and A549 were evaluated by caspase-3 and flow cytometry.
• 5β-spirostanol (2) and a cholestane (17a) induced apoptosis through different mechanisms.
• (22R)-spirosolanol (11) selectively induced apoptosis without affecting caspase-3.
Steroidal glycosides (1–18), including 10 new compounds (1–10), were isolated from the bulbs of Fritillaria meleagris (Liliaceae). The structures of the new compounds were determined by two-dimensional (2D) NMR analysis, and by hydrolytic cleavage followed by spectroscopic and chromatographic analysis. The isolated compounds and their aglycones were evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung adenocarcinoma cells. Morphological observation and flow cytometry analysis showed that 5β-spirostanol glycoside (2) and a cholestane derivative (17a) induced apoptotic cell death in HL-60 cells through different mechanisms of action. Furthermore, the (22R)-spirosolanol glycoside (11) selectively induced apoptosis in A549 cells without affecting the caspase-3 activity level.
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Journal: Steroids - Volume 78, Issue 7, July 2013, Pages 670–682