Three new steroids from the roots of Serratula wolffii

Investigation of the methanol extract of the roots of Serratula wolffii resulted in an ecdysone-related compound, 2β,3β,20R,22R,25-pentahydroxy-5β-cholest-6,8(14)-dien (1), a new ecdysteroid, 24-methylene-shidasterone (2), the known compound stachysterone B (3) and its 14,15-α-epoxide (4), a novel natural product. The structures of compounds 1-4 were established by spectral analysis (1H NMR, 13C NMR, COSY, NOESY, HMQC, HMQC-TOCSY and HMBC).