کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2029066 | 1070470 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Studies on neurosteroids
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The identification and quantification of tetrahydrocorticosterone isomers (THBs; 3α,5α-, 3β,5α-, 3α,5β- and 3β,5β-THB) in rat brains using liquid chromatography (LC)-mass spectrometry (MS) are described. For the identification, the THBs were converted to the atmospheric pressure chemical ionization (APCI)-active derivatives, i.e., the dinitrobezoyl esters and 2-nitro-4-trifluoromethylphenyl hydrazones, and detected in the negative-ion mode. These derivatives showed 60- and 40-fold higher sensitivities, respectively, than intact steroids measured in the positive-APCI-MS. The derivatized THBs were satisfactorily separated from the others during the reversed-phase LC. The THBs were not detected at all in the brains of the unstressed rats. When the rats were exposed to the immobilization for 20 min, 3α,5α- and 3β,5α-THB were detected as the major metabolites together with small amounts of 3α,5β- and 3β,5β-THB in the male rat brain, while only 3α,5α-THB was detected in the female rats. Thus, the steroid variety found in the brains was different between the sexes. In the next step, 3α,5α-THB, a major metabolite found in the brains of the stressed rats, was quantified as its dinitrobezoyl ester. This method was accurate and reproducible, and the limit of quantitation was 1.0 ng/g tissue when a 50 mg tissue sample was used. There was also a sex difference in the brain 3α,5α-THB level; it was significantly higher in the female rats than in the male rats (P < 0.05), although the brain corticosterone level was not higher in the stressed female rats than in the male rats (no statistical difference).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 72, Issue 13, November 2007, Pages 865-874
Journal: Steroids - Volume 72, Issue 13, November 2007, Pages 865-874
نویسندگان
Tatsuya Higashi, Hiroyuki Yokoi, Hitoe Maekubo, Ayako Honda, Kazutake Shimada,