کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2034281 | 1072003 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, Ï2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, Ï2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 38, Issue 1, January 2010, Pages 135-143
Journal: Biologicals - Volume 38, Issue 1, January 2010, Pages 135-143
نویسندگان
Juan Pablo Pilili, Norma V. González, Gabriela Molinari, Miguel A. Reigosa, Sonia Soloneski, Marcelo L. Larramendy,