کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2034281 1072003 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Testing genotoxicity and cytotoxicity strategies for the evaluation of commercial radiosterilized fetal calf sera
چکیده انگلیسی
Effects of 18 commercial lots of fetal calf serum (FCS) after γ-irradiation and their non-irradiated counterparts were comparatively analyzed on CHO-K1 and MDBK MDL1 cells for genotoxicity [sister chromatid exchange (SCE), micronuclei (MNi), and single cell gel electrophoresis (SCGE)], cytotoxicity [cell-cycle progression (CCP), proliferative replication index (PRI), mitotic index (MI), growth promotion (GP), and plating efficiency (PE)], and microbiological properties (mycoplasma and bovine viral diarrhea virus contamination). SCE and SCGE were the most informative end-points for genotoxicity since significant differences were found in 44.4% (P < 0.05-0.001, Student's t-test) and 61.1% (P < 0.05-0.001, χ2 test) samples, respectively. MI was the cytotoxicity assay revealing the greatest variation, showing differences in 66.7% (P < 0.05-0.001, χ2 test) samples. Thus, these three end-points for screening bioproducts such as FCS were found most suitable for detecting potential geno-cytotoxicants in biological samples; their simultaneous use could be strongly recommended.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 38, Issue 1, January 2010, Pages 135-143
نویسندگان
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