کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2042982 1073314 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loss of INCREASED SIZE EXCLUSION LIMIT (ISE)1 or ISE2 Increases the Formation of Secondary Plasmodesmata
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Loss of INCREASED SIZE EXCLUSION LIMIT (ISE)1 or ISE2 Increases the Formation of Secondary Plasmodesmata
چکیده انگلیسی

SummaryPlasmodesmata (PD) transport developmentally important nucleic acids and proteins between plant cells [1, 2 and 3]. Primary PD form during cell division and are simple, linear channels [4 and 5]. Secondary PD form in existing cell walls after cell division and are simple, twinned, or branched [4]. PD function undergoes a marked reduction at the mid-torpedo stage of Arabidopsis embryogenesis [ 6]. Two mutants, increased size exclusion limit (ise)1 and ise2, fail to undergo this transition, and their null mutations are embryonically lethal [ 7 and 8]. We investigated the ultrastructure of PD in early-, mid-, and late-torpedo-stage embryos and in young leaves. Wild-type (WT) embryos contain twinned and branched (T/B) PD at all stages, but ise1 and ise2 embryos contain significantly higher proportions of T/B PD than WT embryos. WT T/B PD formation occurs in a stage- and tissue-specific pattern that is reversed in ise1 embryos. Silencing ISE1 in Nicotiana benthamiana leaves increases the frequency of secondary PD in existing cell walls. Silencing ISE2 increases the proportion of T/B secondary PD formed. Silenced tissues exhibit increased PD-mediated movement of green fluorescent protein tracers. Thus, silencing of ISE1 and ISE2 phenocopies ise1 and ise2 mutant embryos: when wild-type ISE1 and ISE2 functions are lost, de novo production of PD occurs, leading to increased intercellular transport.


► Arabidopsis torpedo-stage embryos contain twinned and branched (T/B) PD
► T/B plasmodesmata (PD) formation is genetically regulated
► Increased T/B PD formation correlates with more cell-to-cell transport in ise1 and ise2 embryos
► Reduced ISE1 and/or ISE2 transcripts increase secondary PD formation and intercellular transport

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 20, Issue 11, 8 June 2010, Pages 989–993
نویسندگان
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