کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2042989 1073314 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Cortical ER Network Limits the Permissive Zone for Actomyosin Ring Assembly
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
The Cortical ER Network Limits the Permissive Zone for Actomyosin Ring Assembly
چکیده انگلیسی

SummaryPrecise positioning of the cellular division plane is important for accurate segregation of genetic material and determination of daughter cell fates. Here we report a surprising connection between division site positioning and the organization of the cortical endoplasmic reticulum (ER). The cortical ER is an interconnected network of flat cisternae and highly curved tubules sharing a continuous lumen [1 and 2]. Stabilization of high curvature by reticulon and DP1 family proteins contributes to formation of tubules [3, 4 and 5]. We show that in the fission yeast Schizosaccharomyces pombe, the ER network is maintained by a set of three membrane proteins: reticulon/Rtn1p, DP1/Yop1p, and a newly identified evolutionarily conserved protein, Tts1p. Cells lacking the ER domain sustained by these proteins exhibit severe defects in division plane positioning as a result of abnormal dispersion of a key regulator of division site selection, Mid1p, along the cell cortex. This triggers delocalized assembly of actomyosin cables and compromises their compaction into a single medially positioned ring. We propose that the cortical ER network restricts the lateral motion of Mid1p and hence generates a permissive zone for actomyosin ring assembly precisely at the cell equator.


► Rtn1p, Yop1p, and a novel protein Tts1p organize the cortical ER in S. pombe
► Disruption of the cortical ER architecture results in division site mispositioning
► Abnormal cell division is due to dispersion of Mid1p along the cell cortex
► Broadly distributed Mid1p triggers delocalized assembly of actomyosin cables

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 20, Issue 11, 8 June 2010, Pages 1029–1034
نویسندگان
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