کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2043397 1073360 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reprogramming after Chromosome Transfer into Mouse Blastomeres
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Reprogramming after Chromosome Transfer into Mouse Blastomeres
چکیده انگلیسی

SummaryIt is well known that oocytes can reprogram differentiated cells, allowing animal cloning by nuclear transfer. We have recently shown that fertilized zygotes retain reprogramming activities [1], suggesting that such activities might also persist in cleavage-stage embryos. Here, we used chromosome transplantation techniques to investigate whether the blastomeres of two-cell-stage mouse embryos can reprogram more differentiated cells. When chromosomes from one of the two blastomeres were replaced with the chromosomes of an embryonic or CD4+ T lymphocyte donor cell, we observed nuclear reprogramming and efficient contribution of the manipulated cell to the developing blastocyst. Embryos produced by this method could be used to derive stem cell lines and also developed to term, generating mosaic “cloned” animals. These results demonstrate that blastomeres retain reprogramming activities and support the notion that discarded human preimplantation embryos may be useful recipients for the production of genetically tailored human embryonic stem cell lines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 19, Issue 16, 25 August 2009, Pages 1403–1409
نویسندگان
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