کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047270 | 1543317 | 2008 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification and characterization of a 38 kDa glycoprotein functionally associated with mating activity of Paramecium primaurelia Identification and characterization of a 38 kDa glycoprotein functionally associated with mating activity of Paramecium primaurelia](/preview/png/2047270.png)
In Paramecium primaurelia mating interactions take place immediately after mixing mating-competent cells of opposite mating types. The cells clump in clusters (mating reaction) and then separate in pairs. Previous results have shown that sialic acid-containing glycoconjugates are present on the cell surface and are involved in mating-cell pairing. In order to identify the sialic acid-containing glycoprotein(s), we first metabolically radiolabelled non-mating-competent cells with d-[6-3H]galactose, and then analyzed the radiolabelled proteins by anion exchange chromatography. We characterized a 38 kDa (gp38) sialic acid-containing glycoprotein and raised the corresponding polyclonal antibody by means of which we localized the antigen at the level of the oral region of non-mating-competent cells and on the ciliary surface of mating-competent cells. Immunoblot analysis of the ciliary protein fraction showed that the anti-gp38 serum interacted with a 38 kDa protein in both mating types I and II cells. We also demonstrated the functional activity of gp38 in the mating reaction by means of anti-gp38 antibody competition assays.
Journal: European Journal of Protistology - Volume 44, Issue 2, 30 May 2008, Pages 81–90