کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047362 | 1543316 | 2008 | 5 صفحه PDF | دانلود رایگان |
Exocytosis of extrusomes, secretory granules found in protozoa, is involved in prey capture by the heliozoon Actinophrys sol. Here, we show that extracellular Ca2+ is necessary for exocytosis and prey capture in A. sol. We found that A. sol could not capture prey cells in a Ca2+-free solution. L-type Ca2+ channel blockers and a calmodulin antagonist also inhibited the capture of prey. These results suggest that Ca2+ influx via L-type Ca2+ channels plays a crucial role in exocytosis in A. sol. Concanavalin A (Con A) also inhibited prey capture, and the inhibition was relieved by the addition of its hapten sugar, α-mannoside, suggesting that Con A-binding glycoconjugates are implicated in exocytosis of extrusomes and the adhesion of prey cells.
Journal: European Journal of Protistology - Volume 44, Issue 3, 1 August 2008, Pages 163–167