کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047482 1073981 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of glucose-6-phosphate transporter as a key regulator functioning at the autophagy initiation step
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Identification of glucose-6-phosphate transporter as a key regulator functioning at the autophagy initiation step
چکیده انگلیسی
Autophagy is a catabolic process involving autophagosome formation via lysosome. However, the initiation step of autophagy is largely unknown. We found an interaction between ULK1 and ATG9 in mammalian cells and utilized the interaction to identify novel regulators of autophagy upstream of ULK1. We established a cell-based screening assay employing bimolecular fluorescence complementation. By performing gain-of-function screening, we identified G6PT as an autophagy activator. G6PT enhanced the interaction between N-terminal Venus-tagged ULK1 and C-terminal Venus-tagged ATG9, and increased autophagic flux independent of its transport activity. G6PT negatively regulated mTORC1 activity, demonstrating that G6PT functions upstream of mTORC1 in stimulating autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 589, Issue 16, 22 July 2015, Pages 2100-2109
نویسندگان
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