کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047686 1074012 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of galectin-3-induced apoptosis of Jurkat cells by both O-glycans and N-glycans on CD45
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Regulation of galectin-3-induced apoptosis of Jurkat cells by both O-glycans and N-glycans on CD45
چکیده انگلیسی


• The role of CD45 in galectin-3-induced apoptosis of Jurkat cells was studied.
• Galectin-3-induced Jurkat cell death was regulated by O-glycans on CD45.
• N-Glycosylation was individually depleted on CD45 by site-directed mutation.
• Removal of N-glycans of CD45 affected galectin-3 binding and apoptosis induction.

Galectin-3 has been reported to induce apoptosis of Jurkat cells through binding receptors such as CD45. CD45RABC is heavily O-glycosylated and N-glycosylated, while CD45RO is only N-glycosylated. In this study, no apoptosis induced by galectin-3 was detected in CD45RO-transfected cells, whereas apoptosis of CD45RABC-transfected cells was observed, implying that O-glycans on CD45 might play roles in galectin-3-induced apoptosis. O-Glycosylation inhibition assay further suggests the role of O-glycans on CD45 in regulation of galectin-3-induced apoptosis. We also found that deglycosylation at N327 of CD45RO resulted in increased binding to galectin-3 without affecting apoptosis, while deglycosylation at N36 or N109 of CD45RO enhanced galectin-3-induced apoptosis. These data demonstrate that galectin-3-induced apoptosis of Jurkat cells is regulated by both O-glycans and N-glycans on CD45.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 24, 11 December 2013, Pages 3986–3994
نویسندگان
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