کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2047697 1074013 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The Schizosaccharomyces pombe Hikeshi/Opi10 protein has similar biochemical functions to its human homolog but acts in different physiological contexts
چکیده انگلیسی


• SpHikeshi/Opi10 interacts with the S. pombe Hsp70 homolog Ssa2.
• SpHikeshi/Opi10 imports Ssa2 in a HeLa-reconstituted nuclear transport system.
• SpHikeshi/Opi10 is not required for cell survival in S. pombe after heat stress.
• SpHikeshi/Opi10 depletion affects gene expression under optimal conditions.
• SpHikeshi/Opi10 is required for cell growth in S. pombe during glucose deprivation.

Human Hikeshi (HsHikeshi) is a nuclear import carrier for Hsp70s and is required for cell survival after heat shock. The Hikeshi homolog in Schizosaccharomyces pombe (SpHikeshi/Opi10) localizes to the nuclear rim, interacts with the Hsp70 homolog Ssa2, and mediates its nuclear import in a reconstituted mammalian nuclear transport system. However, SpHikeshi/Opi10 is not required for heat stress response and survival after heat stress. Instead, SpHikeshi/Opi10 is required for the normal expression of stress response genes under optimal conditions and for cell growth during glucose deprivation. Here, the functions of SpHikeshi/Opi10 are discussed and compared to the functions of HsHikeshi.

Structured summary of protein interactionsSpHikeshi/Opi10physically interacts with Ssa1 by anti tag coimmunoprecipitation (View interaction)SpHikeshi/Opi10physically interacts with Ssa2 by anti tag coimmunoprecipitation (View interaction)

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 10, 21 May 2014, Pages 1899–1905
نویسندگان
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