Identification of an arylalkylamine N-acyltransferase from Drosophila melanogaster that catalyzes the formation of long-chain N-acylserotonins

• Recombinant D. melanogaster AANATL2 was expressed and purified from E. coli.
• AANATL2 was found to catalyze the formation of long-chain N-acylarylalkylamides.
• AANATL2 transcripts were localized to the D. melanogaster thorax-abdomen by RT-PCR.
• Endogenous levels of long-chain N-acylserotonins were quantified from the fly.
• AANATL2 and long-chain N-acylserotonins were co-localized to the thorax-abdomen.

Arylalkylamine N-acyltransferase-like 22 (AANATL2) from Drosophila melanogaster was expressed and shown to catalyze the formation of long-chain N-acylserotonins and N-acydopamines. Subsequent identification of endogenous amounts of N-acylserotonins and colocalization of these fatty acid amides and AANATL2 transcripts gives supporting evidence that AANATL2 has a role in the biosynthetic formation of these important cell signalling lipids.