کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047754 | 1074022 | 2013 | 6 صفحه PDF | دانلود رایگان |

• SLN ablation reduces slippage and back-inhibition of SERCA pump activity.
• SLN ablation increases the efficiency of SERCA pumps in vivo.
• SLN ablation reduces ATP consumption by SERCA pumps in resting soleus muscle.
• Resting muscle and whole body VO2 is not significantly altered by SLN ablation.
The purpose of this study was to examine the effects of sarcolipin (SLN) on sarco(endo) plasmic reticulum Ca2+-ATPase (SERCA pump) energetics in vivo and resting skeletal muscle metabolic rate. Using SLN knockout (Sln−/−) mice we show that SLN ablation increases the transport stoichiometry of SERCA pumps (Ca2+ uptake/Ca2+-ATPase activity) and decreases the relative contribution of SERCA pumps to resting oxygen consumption (VO2) in soleus without affecting soleus or whole body VO2. These data suggest that the lower energy requirements for Ca2+ cycling in resting skeletal muscle of Sln−/− mice do not impact significantly either skeletal muscle or whole body metabolic rate.
Journal: FEBS Letters - Volume 587, Issue 11, 5 June 2013, Pages 1687–1692