کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2047865 | 1074039 | 2012 | 5 صفحه PDF | دانلود رایگان |
NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1β. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-κB dependent manner. Furthermore, the corresponding NF-κB binding sites (nt −1303 to −1292 and −1238 to −1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-κB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.
► NLRP3 expression was induced by TLR stimulation in a NF-κB-dependent manner in murine macrophages.
► The NF-κB binding elements in NLRP3 promoter were identified.
► NF-κB binding to NLRP3 promoter was confirmed with EMSA and ChIP assays in LPS-stimulated macrophages.
Journal: FEBS Letters - Volume 586, Issue 7, 5 April 2012, Pages 1022–1026