کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047867 | 1074039 | 2012 | 6 صفحه PDF | دانلود رایگان |
The dengue virus membrane (M) protein is a key component of the mature virion. Here, we characterised the cellular behaviour of M using a recombinant protein construct to understand its inherent properties. Using confocal microscopy, we showed that M and its intracellular precursor, prM, localised to the endoplasmic reticulum. M protein was also detected on the cell surface and secreted, suggesting that M can enter the secretory pathway. In addition, cross-linking studies showed that M can form dimers and tetramers. These findings suggest that M behaves as a secretory protein analogous to the major envelope protein E.
► Dengue virus PrM and M proteins localise to the endoplasmic reticulum.
► M is secreted from the cells while PrM is not.
► M is able to oligomerize into dimers.
Journal: FEBS Letters - Volume 586, Issue 7, 5 April 2012, Pages 1032–1037