کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047930 | 1074045 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Over-expression of human cytomegalovirus miR-US25-2-3p downregulates eIF4A1 and inhibits HCMV replication Over-expression of human cytomegalovirus miR-US25-2-3p downregulates eIF4A1 and inhibits HCMV replication](/preview/png/2047930.png)
• eIF4A1 is the direct target gene of miR-US25-2-3p.
• Over-expression of miR-US25-2-3p inhibits HCMV replication by targeting eIF4A1.
• Over-expression of miR-US25-2-3p decreases eIF4A1-mediated translation.
• miR-US25-2-3p significantly inhibits host cell proliferation and genomic DNA synthesis.
It has been reported that human cytomegalovirus (HCMV) miR-US25-2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering RNA (siRNA) and miR-US25-2-3p mediated eIF4A1 knockdown experiments revealed that high level of miR-US25-2-3p in MRC-5 cells decreased HCMV and host genomic DNA synthesis, and inhibited cap-dependent translation and host cell proliferation. However, eIF4A1 up-regulation induced by miR-US25-2-3p inhibitor increased HCMV copy number. Therefore, the over-expression of miR-US25-2-3p and consequent lower expression of eIF4A1 may contribute to the inhibition of HCMV replication.
Journal: FEBS Letters - Volume 587, Issue 14, 11 July 2013, Pages 2266–2271