کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2047999 | 1074050 | 2011 | 7 صفحه PDF | دانلود رایگان |
MiR-17-92 cluster miRNAs are disclosed to contribute to the development of multiple organs and tumorigenesis, but their roles in pancreas development remains unclear. In this study, we found that miR-19b, a member of miR-17-92, was highly expressed in the pancreatic progenitor cells, and miR-19b could target the 3′ UTR of NeuroD1 mRNA to decrease its protein and mRNA levels. Functional analysis showed that miR-19b exerted little effect on the proliferation of pancreatic progenitors, whereas it inhibited the expression of insulin 1, but not insulin 2 in MIN6 cells. These results suggest that miR-19b can downregulate insulin 1 expression through targeting transcription factor NeuroD1, and thus regulate the differentiation and function of β-cells.
► Little is known about which miRNAs regulate the proliferation or differentiation of pancreatic progenitor cells.
► We found that miR-19b, a member of miR-17-92, was highly expressed in the pancreatic progenitor cells.
► MiR-19b could target 3′ UTR of NeuroD1 mRNA to decrease its protein and mRNA levels.
► MiR-19b also inhibited insulin 1 expression in pancreatic β-cell due to the reducing of NeuroD1.
► Our results indicate that miR-19b is involved in regulation of β-cell differentiation and function.
Journal: FEBS Letters - Volume 585, Issue 16, 19 August 2011, Pages 2592–2598